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CRISPR Knockout (K.O.) Protocol 

The protocol our lab uses for CRISPR Knockout (Guide Designing, Cloning, Screening, Cell line Transfection and Potential K.O. screening methods) can be found in the document below. (updated on Apr 22, 2020)

Here is an example of the CRISPR K.O. guides designed in our lab:

CRISPR Knockin (K.I.) Protocol

The protocol our lab uses for CRISPR Knockin (Donor Designing, Cloning, Screening, Cell line Transfection and Potential K.I. screening methods) can be found in the document below. (updated on Apr 22, 2020)

The protocol for PCR screening for CRISPR knockins that our lab has developed can be found at the document below.

Here is an example of the Donor plasmid for CRISPR K.I. designed in our lab:

Website for DNA sequence Analysis

Here is a website that is very useful to analysis the DNA chromatography with double peaks:     http://crispid.gbiomed.kuleuven.be/

We summarized instructions and some tips on how to use the website:

The Russell Lab

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